We offer soil nitrate analysis methods (semi-quantitative and quantitative) that are different than other nitrate methods by utilizing an enzyme called Nitrate Reductase (NaR). The simplified Nitrate Test Kits offer easy, reliable, accurate, and non-toxic nitrate testing results. The kits are lightweight and compact, so nitrate testing can be done anywhere on-site. Everything is prepackaged and pre-calculated - once samples are collected, testing is a breeze. Enzyme and Reagent Packs are also available for Discrete Analyzers, laboratory high performance Colorimeters and Continuous Flow Analyzers
The Nitrate Reductase Method is simple. A small soil sample is mixed with buffer containing Nitrate Reductase. The reduction of nitrate to nitrite is accomplished within 20 minutes, then NADH is added and incubated for 10 minutes. The addition of Griess reagents yields a pink color that can be visually compared with a color chart. A color slider app. is available to download to an Andriod device. In laboratories, samples can be analyzed using a spectrophotometer measuring absorbance at 540 ± 20 nm. A standard curve is generated using standards of known nitrate-N content that is used to determine the nitrate-N content of samples.
This video demonstrates the simplified rapid soil nitrate test that can be performed by non-technical personnel in the field.
Above information is courtesy of NECi (The Nitrate Elimination Company, Inc.)